Chemical constituents from pericarps of Aquilaria sinensis and their biological activities
10.7501/j.issn.0253-2670.2013.10.007
- Author:
Xing ZHANG
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
7-methoxy-4'-hydroxyisoflavone;
Antibacterial activity;
Aquilaria sinensis (Lour.) Gilg;
Costunolide;
Cytotoxic activity
- From:
Chinese Traditional and Herbal Drugs
2013;44(10):1248-1252
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents from the pericarps of Aquilaria sinensis and their cytotoxic and antibacterial activities. Methods: The compounds were isolated by various chromatographic methods (silica gel, reverse silica gel, Sephadex LH-20, preparative TLC, and so on) and recrystallization. Their structures were identified by extensive analysis of the spectroscopic data. The cytotoxic effects of these compounds on neuroglioma SF-268, breast cancer MCF-7, large cell lung cancer NCI-H460, and hepatoma carcinoma HepG-2 cell lines were observed in vitro by SRB staining, and the antibacterial effects of these compounds on Staphyloccocus aureus and Escherichia coli were observed in vitro by Resazurin staining method. Results: Ten compounds were isolated from the pericarps of A. sinensis and identified as isorhamnetin 3-O-(6″-O-(Z)-p-coumaroyl)-β-D-glucopyranoside (1), buddlenoid A (2), 7-methoxy-4'-hydroxyisoflavone (3), luteolin (4), trans-p-coumaric acid ethyl eater (5), costunolide (6), epifriedelanol (7), stigmasterol (8), p-hydroxybenzoate (9), and pyrocatechol (10). Conclusion: Compounds 1-3, 5, 6, 9, and 10 are first isolated from the plant and compounds 1-3, 5, and 6 are isolated from the plants of genus Aquilaria Lam. for the first time. Compound 6 exhibits the significant cytotoxic activities against the above four cell lines and compounds 1 and 4 show some antibacterial activities against S. aureus and E. coli.
