Identification of active components in Syngnathus acus by HILIC-ESI-TOF/MS and study on their specific fingerprint chromatograms
10.7501/j.issn.0253-2670.2013.13.026
- Author:
Heng-Qiang ZHAO
1
Author Information
1. Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province
- Publication Type:Journal Article
- Keywords:
Accelerated solvent extraction;
Characteristic fingerprint chromatograms;
Electrospray ionization-time of flight-mass spectrometry;
Hydrophilic interaction chromatography;
Syngnathus acus Linnaeus
- From:
Chinese Traditional and Herbal Drugs
2013;44(13):1836-1841
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a new method based on hydrophilic interaction chromatography-electrospray ionization-time of flight-mass spectrometry (HILIC-DAD-ESI-TOF/MS) for the rapid identification of the active components in Syngnathus acus and the development of their specific fingerprint chromatograms. Methods: Samples were extracted by accelerated solvent extraction, and the extraction conditions were optimized. The developed HILIC-DAD-ESI-TOF/MS method was used to identify the components in water extract from S. acus, and a chromatographic fingerprint based on HILIC analysis was established. Results: Ten compounds in S. acus extract could be primarily identified by HILIC-DAD-ESI-TOF/MS on-line detection, in which seven nucleosides were determined. The HPLC characteristic fingerprint was established on the basis of analysis on the multi batches of S. acus, which could be used to evaluate the quality of S. acus combined with similarity calculation. Conclusion: This method is simple and rapid, and is a powerful tool for the identification of S. acus.
