Absorption of multi-components from Coptidis Rhizoma in Caco-2 monolayer model and their interactions
10.7501/j.issn.0253-2670.2013.13.018
- Author:
Juan QI
1
Author Information
1. Zhejiang Chinese Medical University
- Publication Type:Journal Article
- Keywords:
Apparent permeability coefficient;
Aqueous extract of Coptidis Rhizoma;
Caco-2 monolayer model;
Data fitting;
Multi-component interactions
- From:
Chinese Traditional and Herbal Drugs
2013;44(13):1801-1806
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To research the correlation between compositions and absorption of the multi-components in Coptidis Rhizoma and to explore the methods suitable for identifying the interactions of multi-components in Coptidis Rhizoma during the absorption process. Methods: Samples of various compositions by their different combinations of each component in Coptidis Rhizoma were designed and prepared using Caco-2 monolayer model. Using HPLC method for the simultaneous quantitative determination, taking the inherent apparent permeability coefficients (Papp) by the individual transport of each component as reference, the least square multi-linear regression was used for data fitting to obtain the correlation between interaction intensity of Papp and concentration of each component by their different combinations and to analyze the interaction type and intensity of multi-components during the absorption. Results: The quantitative relationship of multi-component interactions for coptisine, jatrorrhizine, berberine, and palmatine was obtained, respectively. The main contributors for the multi-component interaction, including single component and interactive products of the different components, were also identified. The quantitative relationship affecting the absorption was also obtained. Conclusion: A well-designed method for studying the multi-component interactions of Coptidis Rhizoma using mathematical method is practicable.
