HPLC-DAD fingerprint of Shengxinfa Capsules
10.7501/j.issn.0253-2670.2013.14.013
- Author:
Li-Bin FENG
1
Author Information
1. Guangxi Yulin Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Common peak;
Fingerprint;
HPLC-PADA;
Quality evaluation;
Shengxinfa Capsules;
Similarity
- From:
Chinese Traditional and Herbal Drugs
2013;44(14):1935-1938
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the fingerprint of Shengxinfa Capsules (consisted of Psoraleae Fructus, Polygonui Multiflori Radix, Ligustri Lucidi Fructus, Angelicae Sinensis Radix, Gastrodiae Rhizoma, Polygonati Rhizoma, etc.) for the quality evaluation. Methods: The fingerprint was established by HPLC with Photodiode Array Detector (PADA). The acetonitrile-0.1% phosphoric acid (gradient elution) was employed as the mobile phase, column temperature was at 35°C, flow rate was 1.0 mL/min, and detection wavelength was at 246 nm. Results: There were 13 common peaks appeared in the PADA fingerprint, which were defined by software published by the Chinese Pharmacopeia Committee. The similarity of 11 batches was more than 0.99. Conclusion: The method is simple, accurate, and sensitive. It is suitable to be used for the quality evaluation of Shengxinfa Capsules.
