Cloning and sequence analysis of phytoene desaturase gene in Pogostmen cablin
10.7501/j.issn.0253-2670.2013.17.020
- Author:
Pu-Yue OUYANG
1
Author Information
1. Guangdong Food and Drug Vocational College
- Publication Type:Journal Article
- Keywords:
Bioinformatical analysis;
Gene cloning;
PcPDS1 gene;
Phytoene desaturase;
Pogostemon cablin (Blanco) Benth
- From:
Chinese Traditional and Herbal Drugs
2013;44(17):2446-2452
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In order to elucidate the carotenoid metabolic pathway in Pogostemon cablin, a phytoene desaturase (PDS) of P. cablin (PcPDS1) gene was cloned and analyzed bioinformatically. Methods: The full length PDS gene sequence was retrieved from transcriptomic database of P. cablin, the full length primer was designed for PCR verification, and PcPDS1 gene was analyzed using several bioinformatical softwares. Results: PcPDS1 (NCBI accession, KC854409) contained 1960 bp length of open reading frame (ORF) and encoded 569 amino acids postulated. Additionally, the physical and chemical properties of PcPDS1-coded protein were analyzed by bioinformatical softwares. Phylogenetic analysis showed that PcPDS1 was tightly clustered with PDS genes in Gentiana lutea, Nicotiana tabacum, and Ipomoea batatas, which is consistent to the phylogenetic relationship of species. Conclusion: PcPDS1 is successfully coloned and molecularly characterized.
