Protection of salvianolic acid B on isoproterenol-induced cardiomyocyte hypertrophy of neonatal rats
10.7501/j.issn.0253-2670.2013.17.015
- Author:
Ji-Chao WU
1
Author Information
1. Department of Pathophysiology
- Publication Type:Journal Article
- Keywords:
Cardiomyocyte hypertrophy;
Isoproterenol;
JAK1/STAT 3signaling pathway;
Oxidative stress;
Salvianolic acid B
- From:
Chinese Traditional and Herbal Drugs
2013;44(17):2422-2426
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of salvianolic acid B (Sal B) on isoproterenol (ISO)-induced cardiomyocyte hypertrophy of neonatal rats and clarify the underlying mechanisms. Methods: Hypertrophy in neonatal rat ventricular myocytes was induced by ISO. The effect of Sal B on the myocardial viability of neonatal rats was measured by MTT. The mRNA expression levels of ANP and BNP were detected by RT-PCR. Colorimetric method was employed to measure SOD activity and MDA content. The expression levels of JAK1 and STAT3 were assessed by Western blotting. Results: Sal B at different concentration had no effect on the myocardial viability of neonatal rats. Compared with the model group, Sal B at 10 and 20 μmol/L could obviously down-regulate the gene expression levels of ANP and BNP (P < 0.01, 0.05), significantly increase SOD activity, and decrease MDA content. The protein expression levels of JAK1/STAT3 were down-regulated (P < 0.01, 0.05). Conclusion: Sal B could effectively inhibit ISO-induced cardiomyocyte hypertrophy of neonatal rats and the mechanism may be related with the anti-oxidative stress and the inhibition of JAK1/STAT3 signaling pathway.
