Rapid determination of total flavonoids, total saponins, and soluble solid content in Fufang Ejiao Syrup by NIRS
10.7501/j.issn.0253-2670.2013.17.011
- Author:
Hai-Fan HAN
1
Author Information
1. Pharmaceutical Informatics Institute
- Publication Type:Journal Article
- Keywords:
Fufang Ejiao Syrup;
Near-infrared spectroscopy;
Soluble solid content;
Total flavonoids;
Total saponins
- From:
Chinese Traditional and Herbal Drugs
2013;44(17):2397-2403
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an expeditious method for the simultaneous determination of main active compounds in Fufang Ejiao Syrup (FES, compound Ejiao oral liquid) by near infrared spectroscopy (NIRS) in transflective mode. Methods: The contents of total flavonoids and total saponins were determined by colorimetric assays, while the soluble solid content was determined using a moisture analyzer as reference value. Partial least squares regression (PLSR) was employed for the calibration of regression models between NIRS and the indexes. Results: The correlation coefficients (R2) of the constructed models for total flavonoids, total saponins, and soluble solid content were 0.9798, 0.9660, and 0.9978, respectively, and the root mean square errors of prediction (RMSEP) were 69.6, 17.6 μg/mL, and 0.394%, respectively. Conclusion: The proposed method is fast and accurate, and facilitating the rapid analysis of main active components in FES.
