Research on dose-effect relationship of Wuwei Xiaodu Drink on antibacterial metabolism by MSPQC
10.7501/j.issn.0253-2670.2013.18.015
- Author:
Xiao-Qing ZHANG
1
Author Information
1. Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Bacteriostatic;
Dose-effect relationship;
Minimum bactericidal concentration;
Minimum inhibitory concentration;
MSPQC;
Wuwei Xiaodu Drink
- From:
Chinese Traditional and Herbal Drugs
2013;44(18):2569-2572
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The effect of Wuwei Xiaodu Drink (WXD) on the metabolism of bacteria and the dose-effect relationship were investigated by MSPQC. Methods: The frequency shift-time curves of the growth and metabolism of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa affected by WXD were obtained by using MSPQC. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were gained according to FDT and the frequency shift. The dose-effect relationship was analyzed according to the curve which represented the relationship between the concentration of WXD and FDT. The parallel test was carried out with the traditional tube dilution method. Results: The results indicated that WXD inhibited the growth of E. coli, S. aureus, and P. aeruginosa, which was dose-dependent. The higher the concentration was, the stronger the antibacterial effect became. The MBC of WXD for three kinds of pathogenic bacteria was 0.9 g/mL. The MIC of WXD for S. aureus and P. aeruginosa was 0.8 g/mL, however for E. coli was 0.7 g/mL within 24 h. Conclusion: MSPQC is a sensitive, quantitative, quick method, which could provide the process information in real time for determining the dose-effect relationship of antimicrobial Chinese materia medica.
