Preparation and characterization of gypenosides liposomes consisted by lecithin and stigmasterol
10.7501/j.issn.0253-2670.2013.18.007
- Author:
Fan YU
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
Gypenosides;
Liposomes;
Orthogonal design;
Preparation technology;
Stigmasterol
- From:
Chinese Traditional and Herbal Drugs
2013;44(18):2526-2530
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the feasibility of preparing gypenoside liposomes (GLs) with stigmasterol instead of cholesterol, and to optimize the prescription and preparation of GLs. Methods: GLs without cholesterol were prepared by ethanol injection method with lecithin and stigmasterol, and the encapsulation efficiency was determined by protamine precipitation method. The encapsulation efficiency was adopted to evaluate and optimize the preparation process as response in orthogonal design assay. GLs were characterized by Dynamic Light Scattering and Atomic Force Microscope (AFM) using particle size and electric potential as indexes. Results: Ethanol injection was the best method to prepare GLs with lecithin and stigmasterol. The optimal preparation process determined by orthogonal design assay was as follows: the ratio of gypenosides to lecithin was 1:10, the ratio of lecithin to stigmasterol was 4:1, the pH value of PBS buffer solution was 7.0, and the hydration temperature was 35°C. The entrapment efficiency was (77.00 ± 1.17)%, the particle size was (202.6 ± 3.9) nm, the PDI was 0.108 ± 0.003, the zeta potential was (-28.66 ± 0.86) mV, and the morphology was round observed by AFM. Conclusion: The preparation of GLs with lecithin and stigmasterol was feasible. The GLs prepared by optimum preparation process have high encapsulation efficiency, good morphology, and reproducibility.
