Effects of different pulverization fineness on in vitro dissolution rate of Compound Biejia Ruangan Tablet based on biological value evaluation
10.7501/j.issn.0253-2670.2013.18.006
- Author:
Yun-Zhi XIAO
1
Author Information
1. Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Compound Biejia Ruangan Tablet;
Dissolution rate;
MTT method;
Particle size;
Solid preparation of Chinese materia medica
- From:
Chinese Traditional and Herbal Drugs
2013;44(18):2520-2525
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of different pulverization fineness of solid preparations of Chinese materia medica on their in vitro dissolution, using MTT method combined with HPLC. Methods: Compound Biejia Ruangan Tablet (CBRT) was used as model drug, and MTT method was used to obtain the characteristic cell inhibitory rate by different pulverization fineness of dissolving solutions in the dissolution medium (phosphate buffer, pH value 7.4) at different time points. From these results, the cumulative dissolution of CBRT based on cell inhibitory rate was obtained. The dissolution rates of paeoniflorin was determined by HPLC method. Using f2 similar factor, the relevance of these two methods was evaluated. Results: Dissolution of paeoniflorin is changed with the change of pulverization fineness, the accumulative dissolutions of 200 and 300 meshes in vitro were higher than those of other meshes. The results showed that f2 values of 200 and 300 meshes were more than 50, indicating that there was a good correlation between the two methods of measuring the dissolution rate. Conclusion: The results show that the biological potency detection could be used to screen the particle size of CBRT. Considering the production cost of CBRT, 200 mesh is the best particle size.
