Accumulation of main ginsenoside components in somatic embryogenesis and plantlet regeneration of Panax notoginseng
10.7501/j.issn.0253-2670.2013.19.023
- Author:
Feng-Yang SUN
1
Author Information
1. College of Life Sciences
- Publication Type:Journal Article
- Keywords:
Ginsenosides;
Panax notoginseng (Burk.) F. H. Chen;
Plantlet regeneration;
Somatic embryogenesis;
Total saponins
- From:
Chinese Traditional and Herbal Drugs
2013;44(19):2753-2758
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the accumulation of total saponins and ginsenosides (Rg1, Re, and Rb1) during somatic embryogenesis and plantlet regeneration in Panax notoginseng. Methods: The dynamic accumulation of the total saponins and three components of ginsenosides (Rg1, Re, and Rb1) was analyzed by visible spectrophotometry and high-performance liquid chromatography methods, respectively. Results: The content of total saponins increased from the minimum at embryogenic callus (2.47%) to the maximum at plantlets (7.79%) during somatic embryogenesis development. Because the different specific growth rate of different materials, total saponins accumulation efficiency sort order: plantlets > earlier embryos > later embryos > embryogenic callus. We also found that the total content of the three ginsenosides (Rg1, Re, and Rb1) reached the maximum (7.05 mg/g) in the later embryos, and the minimum (2.78 mg/g) was in the embryogenic callus. The contents of ginsenosides Rg1 and Rb1 reached their maximum (2.24 and 4.03 mg/g) respectively in the earlier and later embryos, and the maximum (1.21 mg/g) of ginsenoside Re content was in embryogenic callus. The accumulation (1.947 4 mg/g) of the three ginsenosides minimum occurred in plantlets, and the maximum (5.022 5 mg/g) reached in earlier embryos. Conclusion: Both total saponin contents and accumulation efficiency reach the maximum in plantlets. Three ginsenoside contents and accumulation reach the maximum in later and earlier embryos, respectively.
