Effect of flavonoid from Glycyrrhizae Radix on small intestine epithelium IEC-6 cell migration and intracellular polyamine content
10.7501/j.issn.0253-2670.2013.19.017
- Author:
Ru-Liu LI
1
Author Information
1. Piwei Institute
- Publication Type:Journal Article
- Keywords:
Cell migration;
Flavonoid from Glycyrrhizae Radix;
Intestinal epithelial IEC-6 cells;
Polyamine;
Polyamine-dependent signaling pathway
- From:
Chinese Traditional and Herbal Drugs
2013;44(19):2722-2726
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of flavonoid from Glycyrrhizae Radix (FGR) on the migration of small intestinal epithelial cells (IEC-6) and intracellular polyamines content. Methods: The cell migration model was constructed by scratch method. Under the intervention of α-difluoromethylornithine (DFMO), the specific inhibitor of polyamine synthesis, or 4-aminopyridine (4-AP), the specific inhibitor of potassium channel, the effect of FGR on cell migration was observed. The intracellular polyamine content being cultured for 12 or 24 h in normal and DFMO intervention condition was detected by precolumn derivatization-HPLC. Results: FGR could reverse the inhibitory effects of DFMO and 4-AP on cell migration, increase the intracellular content of polyamines after 12 or 24 h treatment, and reserve the decrease of polyamine content caused by DFMO. Conclusion: FGR could affect the polyamine-dependent signaling pathway, which may be related to the mechanisms for FGR promoting cell migration.
