Purification of total flavonoids from diaphragma of Juglans regia by macroporous resin
10.7501/j.issn.0253-2670.2013.19.011
- Author:
Guo-Jun WANG
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
Adsorption kinetics characteristics;
Diaphragma of Juglans regia;
Macroporous resin;
Purification technology;
Total flavonoids
- From:
Chinese Traditional and Herbal Drugs
2013;44(19):2688-2692
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the purification technology of total flavonoids from the diaphragma of Juglans regia by macroporous resin. Methods: Twelve macroporous resins were chosn with static and dynamic adsorption and desorption experiments to optimize the purification parameters. Results: AB-8 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH value of 4.70, sample mass concentration of 0.713 5 mg/mL, the loaded amount of 4 mL/g, and loading flow rate of 1.5 BV/h. The sample was eluted by water with 2 BV and 50% ethanol of 2.5 BV, respectively. The purity of total flavonoids increased to 72.25% after the purification, and the yield was 93.94%. Conclusion: AB-8 is an ideal resin with the best enrichment for separating and purifying the total flavonoids from diaphragma of J. regia.
