Determination of nine components in Forsythia Folium collected in different periods
10.7501/j.issn.0253-2670.2013.22.024
- Author:
Xu-Ran ZHI
1
Author Information
1. Department of Pharmaceutical Analysis
- Publication Type:Journal Article
- Keywords:
Forsythia Folium;
Forsythiaside;
HPLC-MS;
Phillygenin;
Phillyrin
- From:
Chinese Traditional and Herbal Drugs
2013;44(22):3231-3235
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC-MS method for the determination of phillyrin, phillygenin, forsythiaside A, forsythiaside F, rutin, quercetin, isoquercitrin, chlorogenic acid, and (+)-epipinoresinol-4'-O-glucoside in Forsythia Folium. Methods: The samples were separated on a Dikma Diamonsil C18 column (150 mm × 4.6 mm, 5 μm), eluted with methanol and water containing 0.02% formic acid in a gradient elution at a flow rate of 0.8 mL/min, and the injection volume was 10 μL. The column temperature was set at 30°C. The multiple-reaction monitoring scanning was employed for the quantification with switching electrospray ion source polarity in negative mode. The ion spray voltage was set at -4500 V and the turbo spray temperature was maintained at 650°C. Results: The regression equations showing linear relationships between peak areas and contents of each compound were obtained. The average recoveries of the compounds ranged from 97.20% to 101.2% and the precision in terms of RSD was in the range of 0.54%-1.24%. Conclusion: The method is sensitive and suitable for the determination of the nine components in Forsythia Folium. As a result, the suitable harvesting time for Forsythia Folium is May.
