Genetic diversity of germplasm resources of Gentiana straminea from Tibet of China
10.7501/j.issn.0253-2670.2013.22.021
- Author:
Liang-Hong NI
1
Author Information
1. College of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
Genetic diversity;
Gentiana straminea Maxim.;
Germplasm resources;
NrDNA ITS;
Traditional Tibetan medicine
- From:
Chinese Traditional and Herbal Drugs
2013;44(22):3212-3215
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the genetic diversity of 17 samples of Gentiana straminea from different habitats of Tibet. Methods: Sampling in populations was carried out, and the related species G. crassicaulis from the same area was as the out group; nr DNA ITS sequences were amplified and analyzed; UPGMA systematic tree was constructed. Results: One polymorphic site of G. straminea from Tibet was found; the six samples of out group have the identical DNA ITS sequences. Conclusion: The difference between G. straminea and the out group is obvious; seventeen samples of G. straminea from different habitats are divided into two groups; genetic diversity is more abundant; the result could provide the basic reference for the quality evaluation and the construction of core germplasm of G. straminea in Tibet.
