Effects of serum containing Dahuang Fuzi Decoction on JAK2/STAT3 signal pathway in mice with severe acute pancreatitis
10.7501/j.issn.0253-2670.2013.22.018
- Author:
Li WU
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Dahuang Fuzi Decoction;
IL-6;
JAK2/STAT3 signal pathway;
Peritoneal macrophage;
Severe acute pancreatitis;
TNF-α
- From:
Chinese Traditional and Herbal Drugs
2013;44(22):3195-3199
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of serum containing Dahuang Fuzi (Rhubarb and Aconite) Decoction (DFD) on JAK2/STAT3 signal pathway in mice with severe acute pancreatitis (SAP). Methods: SAP model in mice was constructed, and then the peritoneal macrophages were vaccinated into the culture plate to set model group, serum containing DFD groups (2.5%, 5%, and 10%), AG490 (10 μmol/L ) positive group, and peritoneal macrophages of normal mice acted as normal control group. After an incubation of 2 h, cells were added with serum containing DFD at different concentration or AG490 0.5 mL, 24 h later, the concentration of TNF-α and IL-6 in supernatant were determined by quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kits, mRNA and protein expression levels of JAK2 and STAT3 in cells were evaluated by Western blotting. Results: DFD could significantly decrease the levels of cytokines TNF-α and IL-6 in the supernatants, inhibit the mRNA and protein expression levels of JAK2 and STAT3 in peritoneal macrophages of SAP mice. Conclusion: DFD could inhibit the JAK2/STAT3 signal pathway and inflammatory responses in peritoneal macrophages of SAP mice.
