Effect of epimedium water extract on osteogenic differentiation in bone marrow derived mesenchymal stem cells of rats and its mechanism
10.7501/j.issn.0253-2670.2013.22.016
- Author:
Li YANG
1
Author Information
1. Pharmacy College of Jinan University
- Publication Type:Journal Article
- Keywords:
BMP-2;
Epimedium water extract;
Mesenchymal stem cells;
Osteogenic differentiation;
TGF-β1
- From:
Chinese Traditional and Herbal Drugs
2013;44(22):3182-3187
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of epimedium water extract (EWE) on the ability of osteogenic differentiation and the expression of TGF-β1 and BMP-2 in inducing mesenchymal stem cells (MSCs) differenting into osteoblast and to further explain its mechanism. Methods: MSCs were isolated and purified by differential time adherent method; the most effective concentration of EWE on the osteogenic differentiation of MSCs was confirmed by the activity of alkaline phosphatase (ALP) and positive rates of ALP staining; according to the differently induced condition, MSCs were divided into four groups: control, classic (induced by the classic osteoblast-induced system), EWE (induced by the most effective concentration of EWE on the osteogenic differentiation), and EWE + classic (induced by the combination of classic osteoblast-induced system and the most effective concentration of EWE on osteogenic differentiation) groups. ALP, type I collagen (Col I), bone gla protein (BGP), and calcium nodes in each group were detected and compared to indicate the osteogenic differentiation of each group. TGF-β1 and BMP-2 in each group were detected by ELISA. Results: The most effective concentration of the EWE on the osteogenic differentiation of MSCs was 500 μg/mL. The classic, EWE, and EWE + classic groups could promote the osteogenic differentiation of MSCs and increase the expression of TGF-β1 and BMP-2. Conclusion: The EWE could promote the osteogenic differentiation of MSCs. The increase of the expression of TGF-β1 and BMP-2 in the induced groups of EWE may be the mechanism of improving the differentiation of MSCs into osteoblast.
