Determination of nine components in Reduning Injection by quantitative analysis of multi-components with a single-marker
10.7501/j.issn.0253-2670.2013.22.013
- Author:
Ya-Fei ZHANG
1
Author Information
1. School of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Caffeic acid;
Chlorogenic acid;
HPLC;
Jasminoidin;
Quantitative analysis of multi-components with a single-marker;
Reduning Injection;
Relative correlation factors;
Secoxyloganin
- From:
Chinese Traditional and Herbal Drugs
2013;44(22):3162-3169
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quantitative analysis of multi-components with a single-marker (QAMS) method for the determination of the nine components in Reduning Injection (RI), comparing with the external standard method, and to evaluate the adaptation and application of QAMS method in the quality control of RI. Methods: Chlorogenic acid, isochlorogenic acid A, and gardenoside were used as the internal reference substances. The relative correlation factors (f) of neochlorogenic acid, cryptochlorogenic acid, and caffeic acid to chlorogenic acid, isochlorogenic acid B and isochlorogenic acid C to isochlorogenic acid A, and secoxyloganin to gardenoside were calculated and established. The results were compared with those obtained by the external standard method. Results: Within the linear ranges, the f values of chlorogenic acid to neochlorogenic acid, cryptochlorogenic acid, and caffeic acid were 0.965, 0.991, and 0.555, respectively; isochlorogenic acid A to isochlorogenic acid B and isochlorogenic acid C were 1.283 and 1.129, respectively; jasminoidin to secoxyloganin was 1.030. The two methods did not show the significant difference in assay results. Conclusion: The QAMS method is feasible and credible, and could be used to determine the multiple components in RI.
