Preparation technology for phospholipids complex of total flavonoids from leaves of Diospyros kaki
10.7501/j.issn.0253-2670.2013.22.009
- Author:
Sheng-Hai ZHANG
1
Author Information
1. School of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
Differential scanning calorimetry;
Phospholipids complex;
Preparation technology;
Total flavonoids from leaves of Diospyros kaki;
X-ray diffraction
- From:
Chinese Traditional and Herbal Drugs
2013;44(22):3136-3140
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence factors in the preparation process for phospholipids complex of total flavonoids from the leaves of Diospyros kaki (PC-TF-LDK) and to optimize the conditions for the formation. Methods: The solvent method was used to prepare the PC-TF-LDK, and the optimized conditions were obtained by means of single factor test and orthogonal design test with the complex ratio as evaluation criterion. The formation of the complex was analyzed by the X-ray diffraction and differential scanning calorimetry. Results: The formation of the PC-TF-LDK was greatly influenced by the ratio of solvents, TF-LDK, and soybean phospholipid. The optimized preparation conditions for the PC-TF-LDK were obtained as follows: the solvent was anhydrous ethanol, the ratio of TF-LDK to soybean phospholipid was 1:2, the mass of reaction concentration was 30 mg/mL, temperature was 30°C, and reaction time was 1 h. Under this condition, 96.95% complex ratio was achieved. Conclusion: The preparation technology for PC-TF-LDK is reliable, stable, and available for industrial production.
