Comparative study on quality of Astragali Radix by two different planting patterns in major producing areas
10.7501/j.issn.0253-2670.2013.23.023
- Author:
Guo-Jun DU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao;
Fingerprint;
HPLC-ELSD;
Metabolomics;
Multivariate statistic analysis
- From:
Chinese Traditional and Herbal Drugs
2013;44(23):3386-3393
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the chemical constituents in traditional planted and two-year-old cultivated Astragali Radix, and to discuss the chemical differences. Methods: The HPLC-ELSD fingerprint of Astragali Radix was collected, and the contents of astragaloside, calycosin-7-O-β-D-glucoside, total polysccharide, and extract were also determined. The similarity analysis, metabolomic multivariate statistical analysis, and quality comparison were performed. Results: The contents of calycosin-7-O-β-D-glucoside and total polysaccharide in traditional planted Astragali Radix were obviously higher than those in two-year-old cultivated Astragali Radix. But the contents of astragaloside and extract showed no difference. The two kinds of Astragali Radix could be distinguished by HPLC-ELSD metabolomic analysis. The differential metabolites were calycosin-7-O-β-D-glucoside, ononin, peaks 4, 5, 13, astragalosides I and II. Conclusion: HPLC-ELSD metabolomic approach combined with the content determination of index components could be used for the quality evaluation of traditional planted and two-year-old cultivated Astragali Radix.
