Effect of Biejiajian Pill on TGF-β1/smad signal pathway in rats with liver fibrosis
10.7501/j.issn.0253-2670.2013.23.019
- Author:
Yu-Feng SUN
1
Author Information
1. Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
α-smooth muscle actin;
Biejiajian Pill;
Hepatic stellate cells;
Liver fibrosis;
Smad3;
Transforming growth factor-β1
- From:
Chinese Traditional and Herbal Drugs
2013;44(23):3364-3367
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the anti-fibrotic mechanism of Biejiajian Pill. Methods: SD rats were randomly divided into six groups: control, model, colchicina (0.1 mg/kg, positive), Biejiajian Pill (0.55, 1.1, and 2.2 g/kg) groups. Except for the control group, the rats in the other groups were sc injected with 40% CCl4 olive oil solution twice a week for consecutive six weeks to establish the models of liver fibrosis.. At the same time, drugs were ig administrated at a volume of 10 mL/kg in each group, once daily for consecutive 11 weeks. At the end of the week 11, all rats were sacrificed. The immunohistochemical technique was applied to analyzing the expression of α-smooth muscle actin (α-SMA) in liver tissue, RT-PCR was applied to analyzing the expression of transforming growth factor-β1 (TGF-β1) protein and smad 3 gene in liver tissue. Results: Compared with the control group, the expression levels of α-SMA, TGF-β1, and smad 3 gene increased significantly (P < 0.05) in the model group. Compared with the model group, Biejiajian Pill and colchicina decreased the expression of α-SMA, TGF-β1 and smad 3 gene, the efficacy of high-dose Biejiajian Pill group was more significantly. Conclusion: The antifibrotic mechanism of Biejiajian Pill may be through lowing the TGF-β1/smad 3 signaling pathways and inhibiting hepatic stellate cells activation and proliferation.
