Chemical constituents in Akebiae Fructus based on UFLC-Q-TOF/MS
10.7501/j.issn.0253-2670.2013.23.006
- Author:
Jiao-Jiao FAN
1
Author Information
1. School of Pharmacy
- Publication Type:Journal Article
- Keywords:
Akebiae Fructus;
Hederagenin;
Oleanane sapogenin;
Triterpenoids;
UFLC-Q-TOF/MS
- From:
Chinese Traditional and Herbal Drugs
2013;44(23):3282-3288
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the chemical constituents in Akebiae Fructus by UFLC-Q-TOF/MS method. Methods: The separation was performed on an Acquity UPLC BEH C18 Column (100 mm × 2.1 mm, 1.7 μm), with a mobile phase using 0.1% formic acid-acetonitrile and water containing 0.1% formic acid (B) for gradient elution. The flow rate was 0.3 mL/min, the temperature of column was 40°C with injection volume of 1 μL. TOF/MS and electrospray ion (ESI) source were applied for the qualitative analysis under the negative ion mode, and the full mass scan range was m/z 100-1500. Results: According to MS principle, twenty-five triterpenoids were identified from the methanol extract of Akebiae Fructus and the chemical structures of other nine unknown compounds were deduced. Conclusion: UFLC-Q-TOF/MS method could identify the main chemical constituents in Akebiae Fructus rapidly and accurately, which lays a foundation for the quality control of Akebiae Fructus.
