Accumulative characteristics of triterpenoid precursor and different triterpenoid branch products in Betula platyphylla
10.7501/j.issn.0253-2670.2013.24.020
- Author:
Xin SU
1
Author Information
1. College of Life Sciences
- Publication Type:Journal Article
- Keywords:
Accumulative characteristics;
Betula platyphylla Suk.;
Betulinic;
Dammarenediol;
Flavonoids;
Oleanolic acid;
Secondary metabolism;
Total triterpenoid
- From:
Chinese Traditional and Herbal Drugs
2013;44(24):3534-3539
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Using three-year-old birch (Betula platyphylla) as the experimental material to study the changes of the accumulation of triterpenoid precursor and triterpenoid components in birch, and to analyze the fluctuation characteristics of triterpenoid component accumulation. Methods: Eight compositions, such as the total triterpene, betulin, flavonoids, etc in twig barks and leaves, were analyzed by HPLC and UV spectrophotometer. Results: The accumulative characteristics of each component in triterpenoid substances was significantly different between the twig barks and leaves. During the growing seasons (May-September), the accumulation of 2, 3-oxidized squalene, betulinic acid, betulin, oleanolic acid, dammarenediol, sterol, and total triterpene reached the peak in July-September. Among these, 2, 3-oxidized squalene had a lower content and its highest content was 0.067 mg/g in July. The content of betulinic acid was 8.34 mg/g, while the content of oleanolic acid reached 37.51 mg/g in September. The highest content of dammarenediol was 14.18 mg/g and the total triterpene content was 168.17 mg/g in July. The higher concentration of total triterpene in the leaves was 126.11 mg/g in August, while the contents of 2, 3-oxidized squalene, betulin, and oleanolic acid were very low and not detected. The accumulated peaks of flavonoids in twig barks and leaves were 53.02 and 58.49 mg/g, respectively in May and August. The fluctuation analysis showed that the accumulation of triterpenoid components did not coincide with that of total triterpene in twig barks and leaves,. The total triterpene, dammarenediol, oleanolic acid, and betulinic acid showed the S-shaped curve in twig barks, and the accumulation of betulin showed the inverted "V" curve, the while trend of flavonoid accumulation was contrary to the total triterpene. The trend of the total triterpene accumulation was similar to that of flavonoids, while the trends of dammarenediol and 2, 3-oxidized squalene accumulation were similar. Conclusion: This research identifies the accumulation and distribution characteristics of triterpenoid and flavonoids in the twig barks and leaves of three-year-old birch, and lays the foundation for triterpenoid resources discovering, sustainable use, and metabolic regulation in twig barks and leaves of birch.
