Isolation of endophytes from Panax quinquefolium and screening and identification of strains with antagonistic activity
10.7501/j.issn.0253-2670.2014.22.022
- Author:
Xue-Zhou LIU
1
Author Information
1. College of Traditional Chinese Medicinal Materials, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Antagonistic activity;
Endophyte;
Identification;
Panax quinquefolium L.;
Screening
- From:
Chinese Traditional and Herbal Drugs
2014;45(22):3332-3336
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the resource situation of endophytes in Panax quinquefolium of Jilin province and to select the endophytes with antagonistic effect on pathogenic fungi of P. quinquefolium. Methods: The strains with the antagonistic activity were screened by applying mixed strains method during preliminary screening and adopting the antagonism method of fermentation liquor in re-screening; Besides, the selected endogenous strains were identified via 16 S rDNA and ITS method. Results: Thirty-four endogenous strains were isolated from P. quinquefolium. After preliminary screening and re-screening, six strains with good antagonistic effect on pathogenic fungi of P. Quinquefolium were selected: B3, B11, B17, B23, B24, and F10. Among them, B3 showed an inhibitory effect on seven pathogenic fungi; F10 had a good inhibitory effect on Cylindrocarpon destructans and Alternaria panax, with an inhibitory zone diameter over 35 mm; According to the identification, B3 and F10 were Bacillus methylotrophicus and Penicillium citreonigrum respectively. Conclusion: Endophytes in P. quinquefolium are diversified and there also exist strains with high antagonistic activity, which can be a good source for biocontrol bacterium and fungus of P. quinquefolium diseases.
