Antihepatocarcinoma effect in vitro of pulchinenoside D and mechanisms
10.7501/j.issn.0253-2670.2014.22.015
- Author:
Wen-Hua YUE
1
Author Information
1. College of Pharmacy, Jiangxi University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Antitumor;
Apoptosis;
Liver cancer;
Mitochondrial pathway;
Pulchinenoside D
- From:
Chinese Traditional and Herbal Drugs
2014;45(22):3295-3301
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To research the antihepatocarcinoma effect in vitro of pulchinenoside D and mechanisms. Methods: MTT assay was used to evaluate the proliferative inhibition of pulchinenoside D on hepatoma carcinoma cells (BEL-7402), and the morphology of BEL-7402 cells was also observed; Colony formation method was used to examine colony-forming role by pulchinenoside D on BEL-7402 cells; The morphological changes of drug-induced apoptosis of BEL-7402 were observed by Hoechst 33342 staining; Flow cytometry assay was used to analyze the apoptosis rate and mitochondrial membrane potential changes of BEL-7402 cells; Western blotting was employed to determine the expression levels of Bcl-2/Caspase-3 protein in BEL-7402 cells. Results: MTT assay showed that pulchinenoside D could inhibit the proliferation of BEL-7402 cells, and colony formation method showed that pulchinenoside D could significantly inhibit the colony formation of BEL-7402 cells; Pulchinenoside D (25.00 μg/mL) could significantly induce the apoptosis of BEL-7402; Pulchinenoside D (18.75 and 25.00 μg/mL) could significantly reduce the mitochondrial membrane potential of BEL-7402 cells; Pulchinenoside D (12.50, 18.75, and 25.00 μg/mL) could significantly increase the expression of Caspase-3; Pulchinenoside D (25.00 μg/mL) could significantly down-regulate the expression of Bcl-2. Conclusion: Pulchinenoside D has antitumor effects in vitro; The mechanism is involved in regulation of the mitochondrial pathway apoptosis-related protein expression of Bcl-2 and caspase-3.
