Determination of allantoin in Dioscoreae Rhizoma by LC-MS/MS
10.7501/j.issn.0253-2670.2014.24.023
- Author:
Ya-Feng ZHANG
1
Author Information
1. Xi'an Institute for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
Allantoin;
Dioscoreae Rhizoma;
ESI-;
HPLC-MS/MS;
MRM
- From:
Chinese Traditional and Herbal Drugs
2014;45(24):3627-3629
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC-MS/MS method for determination of allantoin in Dioscoreae Rhizoma. Methods: Determination of LC system used Agilent Eclipse Plus C18 (100 mm × 4.6 mm, 3.5 μm) column and acetonitrile-water (90:10) as mobile phase in linear gradient condition. The flow rate was 0.3 mL/min. The mass spectrometer was operated under the negative ion mode with ESI source. MRM (precursor ion of m/z 156.9 and product ion of m/z 96.7) was used to qualify allantoin. Results: The linear range of the component was 0.12-6.0 μg/mL, r=0.998 5; The average recovery was 101%, and the RSD was 3.2%. Conclusion: The method is sensitive, reliable, and accurate, and can be applied in the quality control of Dioscoreae Rhizoma.
