Inhibition of salidroside on airway remodeling in asthmatic mice by modulating NF-κB/TGF-β1 signaling pathway
10.7501/j.issn.0253-2670.2014.17.016
- Author:
Ming-Yu ZHENG
1
Author Information
1. College of Pharmacy, Yanbian University
- Publication Type:Journal Article
- Keywords:
Airway remodeling;
Asthma;
NF-kB p65;
Salidroside;
TGF-β1
- From:
Chinese Traditional and Herbal Drugs
2014;45(17):2511-2516
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of salidroside on airway remodeling and NF-κB and TGF-β1 signal transduction pathway in asthmatic mice. Methods: Forty female BALB/c mice of clean grade were randomly divided into four groups (n = 10), such as control, egg protein (OVA) asthma, low-, and high-dose salidroside groups. OVA was used to establish the asthmatic mice model, and the left lung tissues of all mice were treated by HE, PAS, and Masson trichrome staining after the last excitation in 24 h. The right lung tissues were detected by enzyme linked immunosorbent assay (ELISA) to observe the expression levels of IL-4, IL-5, and IL-13 in bronchoalveolar lavage fluid (BALF), by RT-PCR to investigate the expression level of TGF-β1 mRNA, and by Western blotting to detect the expression levels of TGF-β1 and NF-κB p65 proteins. Results: Compared with the control group, the inflammatory cell counts and the expression levels of IL-4, IL-5, and IL-13 in asthmatic model group were increased. The expression levels of TGF-β1 mRNA and NF-κB protein from lung tissues were significantly higher than those in the control group (P < 0.01). Compared with the asthma model group, the inflammatory cell counts, the expression levels of IL-4, IL-5, IL-13 in BALF, TGF-β1 mRNA, and NF-κB of p65 protein were significantly decreased in the salidroside intervention groups, with significant differences (P < 0.01). But for indexes mentioned above, there were no statistical significances between the two different treatment groups (P > 0.05). Conclusion: Salidroside might inhibit the development of airway remodeling in asthmatic mice, and the mechanism might be partly achieved through the inhibition of NF-κB/TGF-β1 signal transduction pathway.
