Study on quantitative determination method of flavonoids and terpene lactone components in Dipyridamole Injection
10.7501/j.issn.0253-2670.2014.22.012
- Author:
Feng-Yun JIN
1
Author Information
1. Guiyang College of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Bilobalide;
Dipyridamole Injection;
Flavonoids;
Ginkgolide;
Isorhamnetin;
Kaempferide;
Quercetin;
Terpenoid lactones
- From:
Chinese Traditional and Herbal Drugs
2014;45(22):3279-3283
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for quantitative determination of Dipyridamole Injection. Methods: The flavonoids were determined using Agela C18 column (150 mm × 4.6 mm, 5μm), acetonitrile-0.4% phosphoric acid (gradient) as the mobile phase with flow rate 1.0 mL/min, detection wavelength of 360 nm, column temperature of 35℃. The lactones were determined using Agela C18 column (150 mm × 4.6 mm, 5 μm), methanol-tetrahydrofuran and water (10:75) solution (gradient) as the mobile phase with flow rate 0.8 mL/min, with evaporative light scattering detector, and column temperature of 30℃. Results: Respectively taking 10 batches of Dipyridamole Injection, the contents of seven major components such as quercetin, kaempferide, isorhamnetin, ginkgolide A, ginkgolide B, ginkgolide C, and bilobalide were determined as 12.4, 20.8, 5.5, 7.2, 2.6, 4.7, and 102.0 μg/mL. Conclusion: The established method is with good stability, repeatability, and precision, which conforms to the requirements and can be used for the quality control of Dipyridamole Injection.
