Spectrum-activity relationship on anti-hepatic fibrosis efficacy of Trionycis Carapax
10.7501/j.issn.0253-2670.2014.17.015
- Author:
Yun-Zhi XIAO
1
Author Information
1. 302 Military Hospital of Chinese People's Liberation Army
- Publication Type:Journal Article
- Keywords:
Anti-hepatic fibrosis efficacy;
Fingerprint;
LX-2 hepatic stellate cell;
Principal component analysis;
Spectrum-activity relationship;
Trionycis Carapax
- From:
Chinese Traditional and Herbal Drugs
2014;45(17):2506-2510
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the spectrum-activity relationship between HPLC of Trionycis Carapax from different regions and its anti-hepatic fibrosis efficacy, in order to reveal the "active component group" for anti-hepatic fibrosis efficacy of Trionycis Carapax. Methods: The samples from 12 regions were determined with the HPLC-DAD method. The representative standard fingerprint was calculated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A). The common patten and the principal component analysis (PCA) were established. Using the LX-2 hepatic stellate cell as a hepatic fibrosis model of Trionycis Carapax to study its inhibition on the hepatic fibrosis cells. The spectrum-effect relationship was studied by SPSS 19.0 software. Results: Seven common peaks in the HPLC-fingerprint of Trionycis Carapax were obtained. It was tentatively concluded that peaks 2 and 5 related better to the inhibitory effect of LX-2 cells (OD value) in the seven characteristic peaks. Peak 4 had the strongest correlation. And the quality of Trionycis Carapax was influenced by the concentration of peak 4 obtained by PCA. Conclusion: All the samples could inhibit the proliferation of LX-2 hepatic stellate cell in some extent. There may be a certain relationship between HPLC fingerprint and anti-hepatic fibrosis efficacy. In addition, the research could be used as the quality control method of Trionycis Carapax.
