ISSR analysis on genetic diversity of Anoectochilus roxburghii
10.7501/j.issn.0253-2670.2014.15.021
- Author:
Ying-Zhen HUANG
1
Author Information
1. Institute of Agricultural Bioresource, Fujian Academy of Agricultural Science
- Publication Type:Journal Article
- Keywords:
Anoectochilus roxburghii (Wall.) Lindl;
Cluster analysis;
Genetic diversity;
ISSR;
PCR;
UPGMA method
- From:
Chinese Traditional and Herbal Drugs
2014;45(15):2230-2234
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the germplasm resources of Anoectochilus roxburghii. and the genetic diversity of its spurious breed. Methods: By ISSR-PCR amplification, 24 samples were PCR amplified, and then electrophoresis detected. After marking the electrophoresis strips with "0" and "1" matrix, the genetic distances were calculated by NTSYSpc software, and the dendrogram was constructed by UPGMA. Results: A. roxburghii, A. formosanus, and A. sp. were identified. A. roxburghii with different geographical distribution and morphology had the close genetic relationship. Conclusion: The wild population of A. roxburghii resources have higher genetic diversity, witch could provide a great genetic library for varieties breeding.
