Research on separation and purification of extract in Xiaozheng Pills with macroporous resins
10.7501/j.issn.0253-2670.2014.22.009
- Author:
Chen LI
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Ferulic acid;
Hesperidin;
Macroporous resin;
Total anthraquinone;
Total saponins;
Xiaozheng Pills
- From:
Chinese Traditional and Herbal Drugs
2014;45(22):3266-3270
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the optimal separation and purification of extract in Xiaozheng Pills (EXP). Methods: We chosed saturation ratio, adsorption ratio, and desorption ratio of total saponins, total anthraquinone, ferulic acid, and hesperidin as evaluation indexes to optimize the purification process of EXP using Z score method. Results: Macroporous resin AB-8 had the best adsorption and desorption properties, while static adsorption was superior to dynamic adsorption. In the course of adsorption, the optimum concentration of the sample liquid was 0.5 g/mL equivalent to raw materia1, static adsorption of 24 h and the resin column diameter-height ratio of 1:10. In the course of elution, the resin column chromatography was eluted with 10 BV of 80% EtOH after removing impurities with 14 BV of water by flow rate of 2 BV/h. Conclusion: Macroporous resin AB-8 is suitable to separate and purify EXP.
