Enzymatic extraction process of calycosin glucoside and pormononetin in Astragali Radix with quadratic general rotary unitized design
10.7501/j.issn.0253-2670.2014.18.013
- Author:
Xu-Hong BAO
1
Author Information
1. Gansu College of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
Calycosin glucoside;
Enzymatic hydrolysis;
Extraction;
Formononetin;
Quadratic general rotary unitized design
- From:
Chinese Traditional and Herbal Drugs
2014;45(18):2641-2646
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The technological conditions for enzymatic extraction of calycosin glucoside and formononetin in Astragali Radix were optimized by using quadratic general rotary unitized design. Methods: On the base of single factors experiments, main factors that affecting the extraction efficiency, such as enzyme dosage, enzymatic hydrolysis time, adding water volume, and extraction time were selected. With the content of calycosin glucoside and formononetin as evaluation index, the process of enzymatic extraction of Astragali Radix was optimized by using quadratic general rotary unitized design. Results: The optimum enzymatic extraction process was as follows: enzyme dosage 340 mg, enzymatic hydrolysis time 110 min, adding water volume 19 times, and extraction time 150 min. Under these optimum conditions, the content of calycosin glucoside was 0.25 mg/g, and formononetin was 67.95 μg/g. Conclusion: The optimum enzymatic extraction process of Astragali Radix is stable and feasible.
