Effects of 3'-hydroxy puerarin on improving insulin resistance in 3T3-L1 adipocytes and their mechanisms
10.7501/j.issn.0253-2670.2014.16.014
- Author:
Xiao-Kang WANG
1
Author Information
1. Department of Pharmacology, College of Pharmacy, Jinan University
- Publication Type:Journal Article
- Keywords:
3'-hydroxy puerarin;
3T3-L1 adipocytes;
Insulin resistance;
Peroxysome proliferator-activated receptor gamma;
Protein tyrosine phosphatase 1B
- From:
Chinese Traditional and Herbal Drugs
2014;45(16):2352-2357
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of 3'-hydroxy puerarin on improving insulin resistance in 3T3-L1 adipocytes and their mechanisms. Methods: The proliferation of 3T3-L1 preadipocytes was tested by MTT assay and the differentiation by oil red O staining. The insulin resistance model was induced by dexamethasone. Cellular glucose consumption was determined by GOD-POD assay and the concentration of FFA by colorimetric methods. The expression of PPARγ and PTP1B genes in insulin resistant adipocytes was analyzed by qPCR. The PPARγ-transactivation activity of 3'-hydroxy puerarin was examined by using a hybrid reporter gene assay and the activity of PTP1B by colorimetric methods. Results: Compared with the medium control group, 3'-hydroxy puerarin significantly activated PPARγ at 0.1 and 10 μmol/L (P < 0.05, 0.01), but showed no effect on PTP1B (P > 0.05); increased the proliferation and differentiation of 3T3-L1 preadipocytes at 1-10 μmol/L (P < 0.05, 0.01). Compared with model group, 3'-hydroxy puerarin enhanced cellular glucose consumption in insulin resistant adipocytes both in basic and insulin stimulation states, decreased the production of FFA, and up-regulated the expression of PPARγ gene (P < 0.01), but showed no effect on the expression of PPARγ gene (P > 0.05). Conclusion: 3'-Hydroxy puerarin can improve the insulin resistance via up-regulating the expression of PPARγ.
