Determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules by HPLC
10.7501/j.issn.0253-2670.2014.16.013
- Author:
Hai-Ning WANG
1
Author Information
1. Guizhou Institute for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
Costunolide;
Dehydrocostuslactone;
HPLC;
Liuwei Muxiang Capsules;
Quality standard
- From:
Chinese Traditional and Herbal Drugs
2014;45(16):2349-2351
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. Methods: The determination was performed on VP-ODS (250 mm × 4.6 mm, 5 μm) with column temperature set at 25℃. The mobile phase consisted of methanol-0.4% phosphoric acid water (72∶28) at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm. Results: The linear range of costunolide and dehydrocostuslactone was 203.12-2 031.20 ng and 207.84-2 078.40 ng (r = 0.999 9), with average recovery at 101.1% and 101.2%, RSD = 0.48% and 0.73% (n = 6). Conclusion: The method proved is simple, accurate, reproducible, and specific, and it can be used for the quality control and evaluation of Liuwei Muxiang Capsules.
