Simultaneous determination of seven components in Shenmai Injection by HPLC-MS/MS
10.7501/j.issn.0253-2670.2014.18.010
- Author:
Yin WU
1
Author Information
1. College of Pharmacy, Hebei Medical University
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb1;
Ginsenoside Re;
Ginsenoside Rg1;
HPLC-MS/MS;
Methylophiopogonanone A;
Methylophiopogonanone B;
Ophiopogonin D;
Ophiopogonin D';
Shenmai Injection
- From:
Chinese Traditional and Herbal Drugs
2014;45(18):2625-2630
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneously analyzing seven components (ginsenosides Rg1, ginsenosides Re, ginsenosides Rb1, ophiopogonin D, ophiopogonin D', methylophiopogonanone A, and methylophiopogonanone B) in Shenmai Injection. Methods: Multiple reaction monitoring (MRM) scan mode was used for the quantification of five saponins and two flavones. The seven constituents were separated within 15 min on a Phenomenex Luna C18 column (150 mm × 2.1 mm, 5 μm) using a mobile phase consisted of acetonitrile and 0.03% acetic acid water solution with gradient elution. Results: The linear relationships between the concentration and peak areas of the seven target components were ginsenoside Rg1 Y=15.6 X + 1.63 × 104, ginsenoside Re Y=14 X + 5.36 × 103, ginsenoside Rb1 Y=2.46 X + 4.74 × 103, ophiopogonin D Y=11 X + 9.73 × 103, ophiopogonin D' Y=5.56 X + 1.64 × 103, methylophiopogonanone A Y=3.58 × 103 X + 2.33 × 104, and methylophiopogonanone B Y=4.87 × 103 X + 2.72 × 104, respectively. The precisions, repeatabilities, and stabilities of the method were good for the seven components. The average recovery ranged from 95.3%-104.3%, and the precision in terms of RSD was less than 2.4%. Conclusion: The method is rapid and reliable for the determination of the seven constituents in Shenmai Injection. Among these constituents, ophiopogonin D, ophiopogonin D', methylophiopogonanone A, and methylophiopogonanone B are quantified in the Shenmai Injection for the first time.
