Simultaneous determination of five active components in Danxiong Tongmai Granule by HPLC
10.7501/j.issn.0253-2670.2014.19.012
- Author:
Yong-Ming ZHAO
1
Author Information
1. Department of Pharmacy, Hebei North University
- Publication Type:Journal Article
- Keywords:
Caffeic acid;
Danxiong Tongmai Granule;
Fumalic acid;
Gallic acid;
HPLC;
Hydroxysafflor yellow A;
Salvianolic acid B
- From:
Chinese Traditional and Herbal Drugs
2014;45(19):2793-2796
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule. Methods: The determination was carried out by HPLC, using Thermo ODS-2 Hypersil (250 mm×4.6 mm, 5 μm) chromatographic column. The mobile phase was 0.1% acetic acid solution-methyl alcohol, gradient elution, and UV detection wavelength was set at 286 nm. The column temperature was set at 30 ℃. The injection volume was 10 μL. Results: Under the above chromatographic conditions, there was a good linearity between the absorption peak area and the concentration in the ranges of 3.18-50.88 μg/mL (r=0.9990) for salvianolic acid B, 2.00-32.00 μg/mL (r=0.9995) for fumalic acid, 6.00-96.00 (r=0.9996) for hydroxysafflor yellow A, 0.60-9.60 μg/mL (r=0.9994) for gallic acid, and 1.00-16.00 μg/mL (r=0.9995) for caffeic acid, respectively. Conclusion: This method is simple, fast, accurate, reliable, and reproducible, and it is proper for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule at the same time.
