Influence of Xiaoyan Decoction and decomposed recipe on expression of survivin and caspase-3 in lung cancer A549 cells
10.7501/j.issn.0253-2670.2014.23.015
- Author:
Xiao-Jiang LI
1
Author Information
1. Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Caspase-3;
Lung adenocarcinoma cancer A549 cell;
Survivin;
Xiaoyan Decoction
- From:
Chinese Traditional and Herbal Drugs
2014;45(23):3436-3439
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibition of Xiaoyan Decoction and its decomposed recipe on apoptosis of pulmonary carcinosis A549 cells. Methods: According to the effect, Xiaoyan Decoction is divided into three groups: Qingre Jiedu (clearing away heat-evil and expelling superficial evils) group, Huoxue Huayu group, and Yiqi Fuzheng group. Using CCK 8 method to detect the proliferation activity of lung adenocarcinoma cancer A549 cells. The Western blotting was adopted to observe the influence on protein expression of survivin and caspase-3 of pulmonary carcinosis A549 cells. And the RT-PCR was adopted to observe the influence on survivin and caspase-3 gene expression of pulmonary carcinosis A549 cells. The inhibition of Xiaoyan Decoction and its decomposed recipe on the apoptosis of pulmonary carcinosis A549 cells was investigated. Results: There were obvious inhibitory effects of cancer cell activity in the three groups. Xiaoyan Decoction in the three groups could induce the apoptosis of lung cancer A549 cell line. Its mechanism was the down regulation of survivin expression. And the related with the elevation of caspase-3 expression is not intimate. Conclusion: Xiaoyan Decoction has the antitumor effect by inducing tumor cell apoptosis through the down regulation of survivin expression.
