Determination of three components in Hubai Burn Tincture by HPLC under double UV wavelengths
10.7501/j.issn.0253-2670.2014.20.012
- Author:
Lin-Zhong HOU
1
Author Information
1. Tangshan Gongren Hospital
- Publication Type:Journal Article
- Keywords:
Berberine hydrochloride;
Emodin;
HPLC;
Hubai Burn Tincture;
Polydatin
- From:
Chinese Traditional and Herbal Drugs
2014;45(20):2932-2934
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determining the contents of emodin, polydatin, and berberine hydrochloride in Hubai Burrn Tincture. Methods: The samples were separated on an Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) column by a gradient elution using acetonitrile and 0.1% phosphoric acid aqueous solution as mobile phase at a flow rate of 1 mL/min. The eluate was detected by double wavelengths: 265 nm for berberine hydrochloride and 306 nm for emodin and polydatin. Column teperature was set at 30 ℃. Results: The linear ranges of emodin, polydatin, and berberine hydrochloride were 6.3-200.0, 9.6-308.0, and 5.0-160.0 ng. The average recovery rates of emodin, polydatin, and berberine hydrochloride were 99.91% (RSD = 1.12%), 99.51 % (RSD = 0.84%), and 99.16% (RSD = 1.17%). Conclusion: This method is accurate, specific, and reliable for the quality control of Hubai Burrn Tincture.
