Preparation of lyophilized powder in oral Pueraria flavonoids loaded solid lipid nanoparticles and determination of release rate of its four effective components
10.7501/j.issn.0253-2670.2014.17.009
- Author:
Xiang-Ping MENG
1
Author Information
1. Medical Technology and Engineering College, Henan University of Science and Technology
- Publication Type:Journal Article
- Keywords:
3'-hydroxypuerarin;
Daidzein;
Daidzin;
Effective components;
Lyophilized powder;
Pueraria flavonoids;
Puerarin;
Release rate;
Solid lipid nanoparticles
- From:
Chinese Traditional and Herbal Drugs
2014;45(17):2467-2474
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare the lyophilized powder of Pueraria flavonoids loaded solid lipid nanoparticles (PF-SLN) and determine the dissolution rate of its four effective components: 3'-hydroxypuerarin, puerarin, daidzin, and daidzein. Methods: PF-SLN was prepared by the high pressure homogenization (HPH) technology. The lyophilized formula contained mannitol as cryoprotectant. The release rates of the four effective components from the PF-SLN lyophilized powder as well as the physical mixture were determined, with artificial gastric juice (pH 1.2) as dissolvent. Results: The technical parameters of PF-SLN preparation optimized by orthogonal test were as follows: The ratio and the dosage of lipid-surfactant were 2:1 and 2.0%, PF dosage was 2.5%, and 150 MPa homogeneity was 15 cycles. The optimal PF-SLN lyophilized powder was loosen with the particle size of (517.1 ± 10.3) nm, polydisperse index of 0.484 ± 0.210, and Zeta potential of (-21.91 ± 2.03) mV, respectively. The in vitro accumulated dissolution rates of PF-SLN lyophilized powder were slower than those of the physical mixture. Conclusion: The method employed to prepare PF-SLN lyophilized powder is feasible. PF-SLN lyophilized powder could delay the in vitro dissolution rate notablely. It might be a novel vehicle potentially for nano-drug delivery system of Pueraria flavonoids.
