Effects of total flavonoids from Dracocephalum moldovica on formation of mouse peritoneal macrophage-derived foam cells and study on their mechanisms
10.7501/j.issn.0253-2670.2014.23.014
- Author:
Wen JIANG
1
Author Information
1. The Sixth Clinical Hospital of Xinjiang Medical University
- Publication Type:Journal Article
- Keywords:
Acyl-coenzyme A, cholesterol acyltransferase-1;
Atherosclerosis;
Cholesterol ester;
Foam cell;
Scavenger receptor A;
Total flavonoids from Dracocephalum moldovica
- From:
Chinese Traditional and Herbal Drugs
2014;45(23):3432-3435
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of total flavonoids from Dracocephalum moldovica on the formation of mouse peritoneal macrophage-derived foam cells and their possible mechanisms. Methods: The mouse peritoneal macrophages were induced with oxidized low density lipoprotein (Ox-LDL) to establish foam cell model, and were intervened with different concentration of total flavonoids from D. moldovica. The foam cells were observed by oil red O staining and the contents of cholesterol ester in cells were detected by fluorescence spectrophotometric method. The expression levels of acyl-coenzyme A, cholesterol acyltransferase-1 (ACAT-1) mRNA in cells and scavenger receptor A (SR-A) on cells were determined using real-time quantitative PCR (RT-qPCR) and flow cytometry, respectively. Results: The Ox-LDL-induced formation of foam cells, accumulation of cholesterol ester in cells, and the expression levels of ACAT-1 mRNA in cells and SR-A on cells were significantly inhibited by total flavonoids from D. moldovica, which showed a tendency toward a dose-dependent manner. Conclusion: These data suggest that total flavonoids from D. moldovica could inhibit the formation of macrophage-derived foam cells, which is probably one of the underlying mechanisms of the total flavonoids for clinical treatment of atherosclerosis.
