Determination of hydrochlorothiazide, prazosin hydrochloride, promethazine hydrochloride, reserpine, and nifedipine illegally added into Qingnao Jiangya Tablets by HPLC method
10.7501/j.issn.0253-2670.2014.23.011
- Author:
Yue-Mei PEI
1
Author Information
1. Anhui Institute for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
HPLC;
Hydrochlorothiazide;
Nifedipine;
Prazosin hydrochloride;
Qingnao Jiangya Tablets;
Reserpine;
Romethazine hydrochloride
- From:
Chinese Traditional and Herbal Drugs
2014;45(23):3414-3417
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To set up an HPLC method for the determination of hydrochlorothiazide, prazosin hydrochloride, romethazine hydrochloride, reserpine, and nifedipine illegally added into Qingnao Jiangya Tablets (QJT). Methods: HPLC method was used. Octadecyl silane bonded silica as a filler (APOLLO C18 column, 250 mm × 4.6 mm) was used with the mobile phase consisting of water containing 0.02% phosphoric acid and acetonitrile in gradient mode. The flow rate was 1.0 mL/min, column temperature was 25℃, and detection wavelength was set at UV 251 nm. Results: The calibration curve showed the good linearity for hydrochlorothiazide, prazosin hydrochloride, romethazine hydrochloride, reserpine, and nifedipine in the ranges of 21.24-2 124.0 ng (r = 1.000 0), 16.184-1 618.4 ng (r = 1.000 0), 19.72-1 972.0 ng (r = 1.000 0). 18.976-1 897.6 ng (r = 1.000 0), and 22.504-2 250.4 ng (r = 1.000 0), respectively; The average recovery rates (n = 6) were 100.93%, 101.61%, 103.07%, 97.58%, and 96.36%, respectively; RSD values were 1.52%, 1.21%, 1.08%, 0.73%, and 0.48%, respectively. Conclusion: The accurate and reproducible method can be used for the determination of hydrochlorothiazide, prazosin hydrochloride, romethazine hydrochloride, reserpine, and nifedipine illegally added into QJT.
