Preparation of nanosuspension-based gel of Ganoderma lucidum triterpenoids and its in vitro transdermal diffusion characteristics
10.7501/j.issn.0253-2670.2014.19.008
- Author:
Cheng-Ying SHEN
1
Author Information
1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Ganoderma lucidum triterpenoids;
Gel;
In vitro dissolution;
Nanosuspension;
Response surface method;
Skin deposition;
Transdermal permeation
- From:
Chinese Traditional and Herbal Drugs
2014;45(19):2770-2775
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare the nanosuspension-based gel of Ganoderma lucidum triterpenoids (GT-NS-gel) and investigate the in vitro transdermal diffusion characteristics. Methods: GT-NS was prepared by high pressure homogenization and then transformed into gel. The formulation of GT-NS-gel was optimized by response surface method with cumulative release of drug from the GT-NS-gel within 24 h, and the amount of drug in the skin after applying GT-NS-gel for 24 h was used as indexes. In vitro percutaneous permeation and skin deposition of GT-NS-gel were studied and compared with those of GT-gel. Results: The GT-NS-gel prepared by optimal formulation (5 mg/g Carbomer 940, 30 mg/g GT, and 47.2 mg/g lecithin) could release in vitro at 24 h to (56.28±2.16)%, and the amount of drug in the skin after applying GT-NS-gel for 24 h was (472.89±8.74) μg/cm2. There was a little deviation between the theoretically predicted value and the measured value. It showed that this model had a good prediction. The amounts of GT penetrating through the skin and in the skin after applying GT-NS-gel for 24 h were (50.73±4.97) and (475.89±10.74) μg/cm2, which were significantly higher than GT-gel (P<0.05). Conclusion: The GT-NS-gel has the ability to increase drug concentration in the skin, which can improve the bioavailability of the local skin.
