Quantitative determination of eight active constituents in Tibetan medicine Zuozhudaxi by HPLC
10.7501/j.issn.0253-2670.2014.15.013
- Author:
Gui-You REN
1
Author Information
1. Ethnic Medicine Instiute of Southwest University for Nationalities
- Publication Type:Journal Article
- Keywords:
Benzoic acid;
Chlorogenic acid;
Clustering analysis;
Costunolide;
Crocin;
Gallic acid;
HPLC;
Kaempferol;
Zuozhudaxi
- From:
Chinese Traditional and Herbal Drugs
2014;45(15):2189-2193
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the simultaneous determination of eight components in Tibetan medicine Zuozhudaxi, and improve the standard of HPLC test. Methods: The HPLC analysis was performed on a Kromasil C18 column (250 mm×4.6 mm, 5 μm). The column temperatrue was set at 30℃. A mixture of methyl alcohol-0.05% phosphoric acid aqueous solution was used as the mobile phase, gradient eluted with the flow rate at 1 mL/min, and detected by different UV wave lengths, i.e. 225 nm for gallic acid, benzoic acid, kaempferol, costunolide, and dehydroepiandrosterone costunolide, 352 nm for chlorogenic acid, and 445 nm for crocin I and crocin II. Results: The eight components were well separated with ideal linear correlations. The average recoveries were in the range of 97.13%-101.42%. The contents of the eight components between two manufactures were different. PLS-DA loading plot of the samples showed that gallic acid, benzoic acid, kaempferol, dehydroepiandrosterone costunolide, and crocin II were the main components which made contributions to the quality of Zuozhudaxi. Conclusion: The method is reliable, stable, accurate, and reproducible for the quantitative determination and quality control of Zuozhudaxi.
