Fingerprint study of Wendan Decoction by UPLC-UV-MS/MS
10.7501/j.issn.0253-2670.2014.23.010
- Author:
Ai-Chao WANG
1
Author Information
1. Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Common peaks;
Eriocitrin;
Fingerprints;
Glycyrrhizic acid;
Hesperidin;
Isonaringin;
Liquiritin;
Naringin;
Neohesperidin;
Poncirin;
UPLC-MS/MS;
Uralsaponin B;
Wendan Decoction
- From:
Chinese Traditional and Herbal Drugs
2014;45(23):3408-3415
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study and establish the UPLC fingerprint of Wendan Decoction (WDD), to ascertain the medicinal source of chromatographic peaks, and to identify the chemical constituents for providing scientific basis in effective substance foundation and quality control of WDD. Methods: The fingerprint of WDD was studied by UPLC-UV, and the similarity threshold of 14 batches of samples of WDD was analyzed; The medicinal source of commom peaks was studied by negative control method; UPLC-MS/MS method was used for the qualitative analysis on chemical constituents in WDD. Results: The UPLC fingerprint of WDD was established, the methodology was examined, and the method showed good precision, stability, and repeatability. The fingerprints of 14 batches of sample were studied and 22 common peaks were signed. The similarity threshold was compared, and it was between 0.863-0.998. Five constituents were identified as hesperidin, neohesperidin, naringin, liquiritin, and glycyrrhizic acid by comparing the retention time and ultraviolet spectra of the peaks with those of the reference substances. The attribution of 22 common peaks was analyzed by comparing with negative control. UPLC-MS/MS method was used for the qualitative analysis on hesperidin, neohesperidin, naringin, liquiritin, and glycyrrhizic acid and inferring eriocitrin, isonaringin, poncirin, and uralsaponin B. Conclusion: The method is stable and reliable, providing a certain reference to substance basis of WDD.
