Simultaneous analysis on 13 components in Yiqi Fumai Injection (freeze-dried) by UPLC-MS/MS method
10.7501/j.issn.0253-2670.2014.23.009
- Author:
Xiao-Li QIAO
1
Author Information
1. Tianjin Nankai Hospital
- Publication Type:Journal Article
- Keywords:
Deoxyschizandrin;
Ginsenosides;
Gomisin A;
Lignans;
Notoginsenoside R1;
Schisandrin B;
Schisandrol B;
UPLC-MS/MS;
Yiqifumai Injection (freeze-dried)
- From:
Chinese Traditional and Herbal Drugs
2014;45(23):3402-3407
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a UPLC-MS/MS analytical method for the simultaneous analysis of ginsenosides (ginsenosides Rb1, Re, Rg1, Rc, Rd, Rf, Rg3, F2, and notoginsenoside R1) and lignans (gomisin A, schisandrol B, deoxyschizandrin, and schisandrin B) in Yiqi Fumai Injection (freeze-dried) (YFI), and measure the contents of these constituents in YFI. Methods: Quantitative research of 13 components in YFI was done by reversed-phase liquid chromatography on a C18 column using a gradient elution (0.1% formic acid in water and 0.1% formic acid in methanol). A triple quadrupole mass spectrometer operating in positive electrospray ionization mode with multiple reaction monitoring was used. Results: Thirteen components in YFI have good linear relationship, precision, stability, and repeatability according to the requirements of the methodology determination. The recoveries were 98.28%-101.08%. The 13 components in three batches of YFI were determined by UPLC-MS/MS method. Conclusion: The developed UPLC-MS/MS method is simple, sensitive, and accurate, and has good repeatability. The 13 components in YFI could be rapidly and accurately quantified by UPLC-MS/MS, which provides the helpful information for the comprehensive quality evaluation of YFI.
