Quality evaluation and control of Polygoni Multiflori Radix based on chemical fingerprint and toxicity monitoring
10.7501/j.issn.0253-2670.2014.23.007
- Author:
Jing-Yao PANG
1
Author Information
1. Traditional Medicament Department, Beijing Friendship Hospital, Capital Medical University
- Publication Type:Journal Article
- Keywords:
Diphenylethylene glycosides;
Fingerprint;
Hepatotoxicity;
High pressure steaming method;
Online shopping;
Polygoni Multiflori Radix;
Polygoni Multiflori Radix Praeparata;
Processing technology;
Quality detection;
Security
- From:
Chinese Traditional and Herbal Drugs
2014;45(23):3392-3396
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Recently hepatotoxicity induced by Polygoni Multiflori Radix Praeparata (PMRP) frequently happened. To aim at dealing with the problems of latest increase of online shopping PMRP and lack of quality monitoring mechanism, the quality and hepatotoxicity of various batches of online shopping PMRP were analyzed, the toxicity-attenuation effects of various processing technologies of Polygoni Multiflori Radix (PMR) were compared, and the reference for clinical safe medication of PMR was provided. Methods: The quality variance of these PMRP was evaluated and the hepatotoxicity-attenuation effects of various processing technologies were compared by adopting index component content determination, chemical fingerprint, and liver cell toxicity evaluation. Results: The content of diphenylethylene glycosides in 26 batches of online shopping PMRP was 0.004%-3.442%, therein eight batches could not conform to Chinese Pharmacopoeia 2010; The chemical fingerprint similarity was between 0.052 and 0.998, therein great differences among six batches existed; The hepatotoxicity difference was significant as 9.7 times, therein the toxicity of four groups of online shopping PMRP samples was higher than that of PMR medicinal material. The toxicity of PMRP attenuated slowly using atmospheric pressure steaming method, and the toxicity by steaming 12 h declined only 13.6%; The toxicity of PMRP attenuated relatively fast by high-pressure steaming and high-pressure black soya bean steaming methods, and the toxicity by steaming 5-6 h declined by 22.1% and tended towards stability. Conclusion: The quality of online shopping PMRP varies greatly and inadequate processing method increases the liver poisoning risk of PMRP to some extent, which indicates that the researches on technology standardization of PMRP should be strengthened. As well, the high-pressure steaming method can ensure the safety of PMRP and shorten the processing time greatly, which should be expected as a promising processing method.
