Identification of metabolites of Pulsatilla saponin D in intestinal microflora of rats in vitro by UPLC-Q-trap-MS
10.7501/j.issn.0253-2670.2014.04.013
- Author:
Hui OUYANG
1
Author Information
1. Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Intestinal microflora;
Metabolites;
Pulsatilla saponin D;
UPLC hybrid triple quadrupole linear ion trap mass spectrometry
- From:
Chinese Traditional and Herbal Drugs
2014;45(4):523-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the metabolites of Pulsatilla saponin D (PSD) in the intestinal microflora of rats in vitro. Methods: The sample of PSD incubated in intestinal microflora of rats in vitro was analyzed by a ultraperformance liquid chromatography/hybrid triple quadrupole linear ion trap mass spectrometry (UPLC-Q-trap-MS). The data were processed with Analyst 1.5 software, and the metabolites of PSD were confirmed by comparing the retention time and MS/MS spectra. The possible structures of metabolites were proposed by analyzing the proposed fragmentation pathway. Results: PSD (M0, parent) and seven metabolites were identified, including hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinosyl (M1), hederagenin 3-O-α-L-glucopyranosyl-(1→4)-α-L-arabinosyl (M2), hederagenin (M3), hydroxylation of PSD (M4), methylation of PSD (M5 and M6), and dehydrogenation of PSD (M7). Conclusion: PSD has a wide range of metabolic in the intestinal microflora of rats in vitro.
