Chemical constituents in leaves of Vaccinium bracteatum and their anti-complementary activity
10.7501/j.issn.0253-2670.2014.04.002
- Author:
Chun-Jun CHU
1
Author Information
1. College of Pharmaceutical Science, Soochow University
- Publication Type:Journal Article
- Keywords:
Anti-complementary activity;
Caffeic acid;
Isoorientin;
Leaves of Vaccinium bracteatum;
Luteolin;
Oleanolic acid;
Ursolic acid
- From:
Chinese Traditional and Herbal Drugs
2014;45(4):458-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents in the leaves of Vaccinium bracteatum and their anti-complementary activity. Methods: The isolation was guided by anti-complementary activity test, compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatographies as well as preparative HPLC. The structures were identified by the various spectroscopic data. The compounds were evaluated for the anti-complementary activity in vitro. Results: Twenty-eight compounds were isolated and identified as pentatriacontane (1), hentriantane (2), glutinone (3), friedelin (4), epifriedelanol (5), lupeol (6), oleanolic acid (7), ursolic acid (8), scopoletin (9), trans-p-hydroxycinnamic acid (10), chrysoeriol (11), apigenin (12), kaempferol (13), maslinic acid (14), corosolic acid (15), euscaphic acid (16), 2α, 3α-dihydroxyurs-12-en-oic acid (17), 19α-hydroxyoleanolic acid (18), caffeic acid (19), isoorientin (20), orientin (21), vitexin (22), isovitexin (23), quercetin-3-O-α-L-rhamnoside (24), isoquerecitrin (25), chrysoeriol-7-O-β-D-glucopyranoside (26), quceritin (27), and luteolin (28). Among them 25 compounds were tested for their anti-complementary activity and 5, 7, 8, 11-13, 15, 18-20, 23-25, 27, and 28 show the various inhibitions on the complement system towards the classical pathway. Conclusion: Compounds 1, 3, 6, 14-18, 23, 25 are obtained from this plant for the first time. Ursolic acid shows the strongest activity in vitro with the CH50 of 0.014 mg/mL.
