Effect of exogenous nitric oxide on seed germination of Oldenlandia diffusa under drought stress
10.7501/j.issn.0253-2670.2014.06.019
- Author:
Zai-Biao ZHU
1
Author Information
1. Institute of Medicinal Plants, Nanjing Agricultural University
- Publication Type:Journal Article
- Keywords:
Drought stress;
Nitric oxide;
Oldenlandia diffusa (Willd.) Roxb.;
Seed germination;
Sodium nitroprusside
- From:
Chinese Traditional and Herbal Drugs
2014;45(6):840-843
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of exogenous nitric oxide (NO) on seed germination of Oldenlandia diffusa under drought stress and to determine the optimal concentration of sodium nitroprusside (SNP, exterior nitric oxide donor) for alleviating the drought stress. Methods: Using 15% PEG6000 to imitate drought stress, the germination potential, germination rate, germination index, and vigor index of O. diffusa seeds were measured by the treatment of SNP at different concentration. Results: The germination process of O. diffusa seeds was significantly suppressed by drought stress. SNP could significantly promote the germination of O. diffusa seeds under drought stress. SNP (0.3 mmol/L) increased the germination rate and germination potential by 2.78- and 7.77-folds, respectively. In the case of germination index and vigor index, SNP (0.1 and 0.3 mmol/L ) showed the maximum positive impact and there was no significant difference between the two SNP concentration treatments. Conclusion: SNP could alleviate the negative influence of drought stress on the germination process of O. diffusa seeds and improve the drought-resistant activity. Among all the treatments, the concentration of SNP at 0.3 mmol/L leads to the maximum germination rate.
