Optimization of decompressing extraction for Salvia mihiorrhiza and its suitability of technology
10.7501/j.issn.0253-2670.2014.06.011
- Author:
Zhen-Feng WU
1
Author Information
1. Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Box-Behnken design;
Decompressing extraction;
Plackett-Burman design;
Response surface;
Salvia mihiorrhiza Bunge;
Salvianolic acid B
- From:
Chinese Traditional and Herbal Drugs
2014;45(6):795-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the rationality of decompressing extraction on Salvia mihiorrhiza and to optimize the decompressing extraction process of salvianolic acid B from S. mihiorrhiza. Methods: HPLC was applied to determining the content of salvianolic acid B. Plackett-Burman design, Box-Behnken design, and response surface analysis were used to screen the main factors and optimize the decompressing extraction process for salvianolic acid B. Results: The optimal decompressing extraction process for salvianolic acid B is that S. mihiorrhiza was immersed in water for 12 h and kept in the dark before extracting, the optimal process parameters were as follows: extraction temperature 80°C, extraction time 89 min, and the ratio of liquid to solid 11. Under this condition, the experimental results for extracted content of salvianolic acid B was 5.01% on average. The deviation rate of predicted value was -1.54% on average. The establishing mathematical model was consistent with the experimental data. Compared with the atmospheric pressure extraction, the content of salvianolic acid B was increased by 28.87% and the dry extract yield was reduced by 9.01%. Conclusion: Using Plackett-Burman design and Box-Behnken design method to optimize the decompressing extraction process of salvianolic acid B from S. mihiorrhiza is scientific and feasible. The method is reliable, accurate, reproducible, and predicted. The decompressing extraction is suitable for the extraction of salvianolic acid B.
